New 3Cs multiplex technique based on CRISPR-Cas technology helps study cancer mutations
Cancer and many other diseases are based on genetic defects. The body can often compensate for the defect of one gene; it is only the combination of several genetic errors that leads to the clinical picture.
The 3Cs multiplex technique based on CRISPR-Cas technology developed at Goethe University Frankfurt now offers a way to simulate millions of such combinations of genetic defects and study their effects in cell culture. These “gene scissors” make it possible to introduce, remove and switch off genes in a targeted manner. For this purpose, small snippets of genetic material (“single guide RNA”) are used as “addresses” that guide the gene scissors to specific sections of the DNA, where the gene scissors then become active.
The scientists from the Institute of Biochemistry II at Goethe University have expanded the 3Cs technique that they developed and patented three…